|Positive WB detected in
|SH-SY5Y cells, HeLa cells, human brain tissue, PC-3 cells
|Positive IHC detected in
|human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:250-1:1000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11928-1-AP targets TMEFF2 in WB, IHC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|TMEFF2 fusion protein Ag2609
|transmembrane protein with EGF-like and two follistatin-like domains 2
|Calculated molecular weight
|374 aa, 41 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Gene expression profiling and real-time PCR analyses identify novel potential cancer-testis antigens in multiple myeloma.
Cancer Cell Int
TMEFF2 promoter hypermethylation is an unfavorable prognostic marker in gliomas.