Filter: AllIHC

IHC staining of human lung using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach cancer using 25581-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 Antibody) at dilution of 1:50 (under 40x lens).

TMEM100 Polyclonal antibody

Name: TMEM100 Polyclonal antibody
Brand: Proteintech
SKU: 25581-1-AP

TMEM100 Polyclonal Antibody for IHC, ELISA

Cat No. 25581-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

Transmembrane protein 100

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in

mouse brain tissue, human brain tissue, human lung tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

25581-1-AP targets TMEM100 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22272 Product name: Recombinant human TMEM100 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-134 aa of BC010128 Sequence: MTEEPIKEILGAPKAHMAATMEKSPKSEVVITTVPLVSEIQLMAATGGTELSCYRCIIPFAVVVFIAGIVVTAVAYSFNSHGSIISIFGLVVLSSGLFLLASSALCWKVRQRSKKAKRRESQTALVANQRSLFA Predict reactive species
Full Name	transmembrane protein 100
Calculated Molecular Weight	134 aa, 14 kDa
GenBank Accession Number	BC010128
Gene Symbol	TMEM100
Gene ID (NCBI)	55273
RRID	AB_2880138
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NV29
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TMEM100 antibody 25581-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Biomolecules Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods Authors - Haofuzi Zhang View Article
human	WB	World J Clin Oncol Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma Authors - Yue-Feng Xu View Article
human	IF	Front Cell Neurosci A novel histone deacetylase inhibitor W2A-16 improves the barrier integrity in brain vascular endothelial cells Authors - Yasuteru Inoue View Article