TMEM100 Polyclonal antibody

TMEM100 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25581-1-AP

Synonyms

Transmembrane protein 100

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Validation Data Gallery

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  IHC staining of human lung using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 25581-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25581-1-AP (TMEM100 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human brain tissue, human lung tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

25581-1-AP targets TMEM100 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM100 fusion protein Ag22272
• Full Name: transmembrane protein 100
• Calculated Molecular Weight: 134 aa, 14 kDa
• GenBank Accession Number: BC010128
• Gene Symbol: TMEM100
• Gene ID (NCBI): 55273
• RRID: AB_2880138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Biomolecules; Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods; Authors - Haofuzi Zhang
human	WB	World J Clin Oncol; Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma; Authors - Yue-Feng Xu
human	IF	Front Cell Neurosci; A novel histone deacetylase inhibitor W2A-16 improves the barrier integrity in brain vascular endothelial cells; Authors - Yasuteru Inoue