TMEM102 Polyclonal antibody

• Featured Product
• KD/KO Validated

TMEM102 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25505-1-AP

Synonyms

CBAP, TMEM102, transmembrane protein 102

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Validation Data Gallery

• 

  WB analysis of HepG2 using 25505-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25505-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25505-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25505-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells, mouse brain tissue
• Positive IHC detected in: human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

25505-1-AP targets TMEM102 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM102 fusion protein Ag22213
• Full Name: transmembrane protein 102
• Calculated Molecular Weight: 508 aa, 54 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC046189
• Gene Symbol: TMEM102
• Gene ID (NCBI): 284114
• RRID: AB_2880107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	J Biol Chem; CBAP regulates the function of Akt-associated TSC protein complexes to modulate mTORC1 signaling; Authors - Wei-Ting Liao ; KD Validated