TMEM102 Polyclonal antibody

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• TMEM102 Polyclonal antibody (25505-1-AP)

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• KD/KO Validated

TMEM102 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25505-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

CBAP, Common beta-chain associated protein, Transmembrane protein 102

• 

  WB analysis of HepG2 using 25505-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 25505-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 25505-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25505-1-AP targets TMEM102 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22213

  Product name: Recombinant human TMEM102 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-260 aa of BC046189

  Sequence: MASAVWGSAPWWGPPPPAPARPLTDIDFCSGAQLQELTQLIQELGVQESWSDGPKPGADLLRAKDFVFSLLGLVHRRDPRFPPQAELLLLRGGIREGSLDLGHAPLGPYARGPHYDAGFTLLVPMFSLDGTELQLDLESCYAQVCLPEMVCGTPIREMWQDCLGPPVPGARDSIHRTESEESSKDWQSSVDQPHSYVTEHEAPVSLEKSPSDVSASESPQHDVVDLGSTAPLKTMSSDVTKAAVESPVPKPSEAREAWPT

• Full Name: transmembrane protein 102
• Calculated Molecular Weight: 508 aa, 54 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC046189
• Gene Symbol: TMEM102
• Gene ID (NCBI): 284114
• RRID: AB_2880107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N9M5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TMEM102 Polyclonal antibody (Cat# 25505-1-AP) has 1 citation. It appears in the Journal of Biological Chemistry (2023), in a study investigating how CBAP regulates Akt-associated TSC protein complexes to modulate mTORC1 signaling. The research field encompasses mTORC1 signaling pathway regulation and protein complex function. The antibody was used for Western blot and immunofluorescence in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	J Biol Chem; CBAP regulates the function of Akt-associated TSC protein complexes to modulate mTORC1 signaling; Authors - Wei-Ting Liao ; KD Validated