TMEM119 Recombinant monoclonal antibody

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  WB analysis using 83448-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83448-6-RR (TMEM119 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83448-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83448-6-RR against Human TMEM119 were performed. The affinity constant is 9.33 nM.

TMEM119 Recombinant Antibody for WB, ELISA

Cat No. 83448-6-RR

Clone No.240433F2

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF, 240433F2

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83448-6-RR targets TMEM119 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: transmembrane protein 119
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_181724.2
• Gene Symbol: TMEM119
• Gene ID (NCBI): 338773
• RRID: AB_3671086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q4V9L6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#66948-1-Ig. The predicted MW of TMEM119 is 29 kDa. Catalog#66948-1-Ig recognizes 50 kDa band may due to glycosylation.