TMEM120A Polyclonal antibody

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• KD/KO Validated

TMEM120A Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 17455-1-AP

Synonyms

NET29, TACAN, TMEM120A, TMPIT, transmembrane protein 120A

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Validation Data Gallery

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  WB analysis of HEK-293 using 17455-1-AP

  WB result of TMEM120A antibody (17455-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM120A transfected HEK-293 cells.

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  WB analysis using 17455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17455-1-AP (TMEM120A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17455-1-AP

  Immunofluorescent analysis of HeLa cells using 17455-1-AP (TMEM120A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, mouse cerebellum tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 2 publications below

Product Information

17455-1-AP targets TMEM120A in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM120A fusion protein Ag10849
• Full Name: transmembrane protein 120A
• Calculated Molecular Weight: 322 aa, 36 kDa, 41 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC029487
• Gene Symbol: TMEM120A
• Gene ID (NCBI): 83862
• RRID: AB_2204630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM120A is a transmembrane protein. The function of TMEM120A has not been widely studied, and is yet to be fully elucidated.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Gain-of-function genetic screening identifies the antiviral function of TMEM120A via STING activation.; Authors - Shuo Li
rat	WB	Biomedicines; Neurodynamic Treatment Promotes Mechanical Pain Modulation in Sensory Neurons and Nerve Regeneration in Rats.; Authors - Giacomo Carta
rat	WB,IF	Arch Oral Biol; Upregulation of TACAN in the trigeminal ganglion affects pain transduction in acute pulpitis; Authors - Yicong Shang
	WB	J Physiol; Regulation of the PKD2 channel function by TACAN; Authors - Xiong Liu
human,rat	WB	Low Urin Tract Symptoms; The expression and distribution of TACAN in human and rat bladders; Authors - Qudong Lu
mouse	WB	Cell Metab; Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain; Authors - Hai-Yan Zhou