Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, mouse cerebellum tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17455-1-AP targets TMEM120A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10849 Product name: Recombinant human TMEM120A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-322 aa of BC029487 Sequence: MQPPPPGPLGDCLRDWEDLQQDFQNIQETHRLYRLKLEELTKLQNNCTSSITRQKKRLQELALALKKCKPSLPAEAEGAAQELENRMKERQGLFFDMEAYLPKKNGLYLSLVLGNVNVTLLSKQAKFAYKDEYEKFKLYLTIILILISFTCRFLLNSRVTDAAFNFLLVWYYCTLTIRESILINNGSRWAGRALREGSMEWGARTLREAGTGAGGDGGSLLQDQRLVGVPSLRVHLPVGSHADVVRRSHVPEIPEPIPLLFHVPELRAVSPVLLPERLPLPPAGAGRAAHHGPHCGGLPVLDVAGPHLPAAFSFLWTLLAAF Predict reactive species |
| Full Name | transmembrane protein 120A |
| Calculated Molecular Weight | 322 aa, 36 kDa, 41 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC029487 |
| Gene Symbol | TMEM120A |
| Gene ID (NCBI) | 83862 |
| RRID | AB_2204630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXJ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMEM120A (also known as TACAN) is a nuclear envelope transmembrane protein essential for adipocyte differentiation and lipid metabolism. Originally identified through proteomic screening, it regulates spatial genome organization by repositioning pro-adipogenic genes from the nuclear periphery to the interior during adipogenesis. Adipocyte-specific knockout mice exhibit lipodystrophy with insulin resistance. TMEM120A was initially proposed as a mechanosensitive ion channel involved in mechanical pain sensing; however, subsequent structural studies revealed it contains a coenzyme A-binding site and shares homology with fatty acid elongases, challenging its role as an ion channel (PMID: 26024229; 34409941; 34374645).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TMEM120A antibody 17455-1-AP | Download protocol |
| WB protocol for TMEM120A antibody 17455-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TMEM120A Polyclonal antibody (Cat# 17455-1-AP) has 6 citations published in Cell Metabolism, Nature Communications, The Journal of Physiology, Biomedicines, Archives of Oral Biology, and Lower Urinary Tract Symptoms. Research fields span metabolic regulation and nutritional stress, antiviral immunity via STING signaling, ion channel function, pain modulation and nerve regeneration, oral pathology, and bladder physiology. Applications cited include Western blotting and immunofluorescence in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Cell Metab Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain | ||
Nat Commun Gain-of-function genetic screening identifies the antiviral function of TMEM120A via STING activation. | ||
Biomedicines Neurodynamic Treatment Promotes Mechanical Pain Modulation in Sensory Neurons and Nerve Regeneration in Rats. | ||
Arch Oral Biol Upregulation of TACAN in the trigeminal ganglion affects pain transduction in acute pulpitis | ||
Low Urin Tract Symptoms The expression and distribution of TACAN in human and rat bladders |
