TMEM135 Polyclonal antibody

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  WB analysis using 31327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31327-1-AP (TMEM135 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TMEM135 Polyclonal Antibody for WB, ELISA

Cat No. 31327-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

transmembrane protein 135, PMP52

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31327-1-AP targets TMEM135 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34057

  Product name: Recombinant human TMEM135 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 352-458 aa of BC051462

  Sequence: YLASKLVETMYFKGIEAGKVPYFPHADTIIYSISTAICFQAAVMEVQTLRPSYWKFLLRLTKGKFAVMNRKVLDVFGTGASKHFQDFIPRLDPRYTTVTPELPTEFS

• Full Name: transmembrane protein 135
• Calculated Molecular Weight: 458 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC051462
• Gene Symbol: TMEM135
• Gene ID (NCBI): 65084
• RRID: AB_3669944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q86UB9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM135, also known as PMP52, contains 3 N-terminal transmembrane domains and 4 C-terminal transmembrane domains. TMEM135 is up-regulated in bone marrow-derived mesenchymal stem cells during differentiation into adipocytes compared to osteoblasts(PMID: 18637193).