TMEM143 Polyclonal antibody

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  WB analysis using 31917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31917-1-AP (TMEM143 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 31917-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31917-1-AP (TMEM143 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 31917-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31917-1-AP (TMEM143 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM143 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31917-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

UNQ5922/PRO19813, Transmembrane protein 143

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Tested Applications

• Positive WB detected in: HH cells, HepG2 cells, Jurkat cells, Ramos cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31917-1-AP targets TMEM143 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM143 fusion protein Ag36277
• Full Name: transmembrane protein 143
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: BC016919
• Gene Symbol: TMEM143
• Gene ID (NCBI): 55260
• RRID: AB_3670142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96AN5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane protein 143 (TMEM143) is a transmembrane protein and a member of the TMEM (Transmembrane) protein family, which is located in mitochondria (PMID: 11256614). It is known that members of the TMEM family play an important role in the development and metastasis of cancer, especially in conveying information between the extracellular environment and cytoplasmic proteins (PMID: 31454669).