TMEM161A Polyclonal antibody

TMEM161A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24898-1-AP

Synonyms

AROS 29, TMEM161A, transmembrane protein 161A

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Validation Data Gallery

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  WB analysis of HEK-293 using 24898-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24898-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24898-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 24898-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24898-1-AP (TMEM161A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24898-1-AP (TMEM161A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24898-1-AP is TMEM161A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM161A fusion protein Ag18974
• Full Name: transmembrane protein 161A
• Calculated Molecular Weight: 479 aa, 54 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC005210
• Gene Symbol: TMEM161A
• Gene ID (NCBI): 54929
• RRID: AB_2879785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM161A also named as transmembrane protein 161A is a 479 amino-acid protein, which belongs to TMEM161 family. The molecular weight of TMEM161A is 56 kDa. TMEM161A is multi-pass membrane protein and localizes on the cell membrane. It may involve in the negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage and cellular response to oxidative stress.