TMEM161A Polyclonal antibody

TMEM161A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24898-1-AP

Synonyms

UNQ582/PRO1152, Transmembrane protein 161A, AROS-29, AROS 29, Adaptive response to oxidative stress protein 29

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Validation Data Gallery

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  WB analysis using 24898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24898-1-AP (TMEM161A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24898-1-AP (TMEM161A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24898-1-AP targets TMEM161A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM161A fusion protein Ag18974
• Full Name: transmembrane protein 161A
• Calculated Molecular Weight: 479 aa, 54 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC005210
• Gene Symbol: TMEM161A
• Gene ID (NCBI): 54929
• RRID: AB_2879785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM161A also named as transmembrane protein 161A is a 479 amino acid protein which belongs to TMEM161 family. The molecular weight of TMEM161A is 56 kDa or 42 kDa. TMEM161A is a multi-pass membrane protein and localizes to the cell membrane. It may be involved in the negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage and cellular response to oxidative stress.