TMEM165 Monoclonal antibody

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  WB analysis of LNCaP using 68584-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 68584-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 68584-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68584-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

TMEM165 Monoclonal Antibody for WB, ELISA

Cat No. 68584-1-Ig

Clone No.3B11C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165, Transmembrane protein PT27

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Tested Applications

• Positive WB detected in: Saos-2 cells, U-251 cells, LNCaP cells, K-562 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68584-1-Ig targets TMEM165 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14872

  Product name: Recombinant human TMEM165 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 61-177 aa of BC003545

  Sequence: VSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHC

• Full Name: transmembrane protein 165
• Calculated Molecular Weight: 324 aa, 35 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC003545
• Gene Symbol: TMEM165
• Gene ID (NCBI): 55858
• RRID: AB_3085284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9HC07
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.