Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, SH-SY5Y cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19925-1-AP targets TMEM175 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13890 Product name: Recombinant human TMEM175 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 222-313 aa of BC005158 Sequence: YVSKVTGWCRDRLLGHREPSAHPVEVFSFDLHEPLSKERVEAFSDGVYAIVATLLILDICEDNVPDPKDVKERFSGSLVAALSATGPRFLAY Predict reactive species |
| Full Name | transmembrane protein 175 |
| Calculated Molecular Weight | 504 aa, 56 kDa |
| Observed Molecular Weight | 41-45 kDa, 54-60 kDa |
| GenBank Accession Number | BC005158 |
| Gene Symbol | TMEM175 |
| Gene ID (NCBI) | 84286 |
| RRID | AB_10666166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BSA9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM175 antibody 19925-1-AP | Download protocol |
| WB protocol for TMEM175 antibody 19925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TMEM175 antibody (Cat# 19925-1-AP) has 27 citations across 24 journals, including Nature, Cell, PNAS, Neuron, Molecular Cell, EMBO Reports, and Advanced Science. Research fields span Parkinson's disease and synucleinopathies, lysosomal K⁺ channel biology, autophagy and mitochondrial dysfunction, neurodegeneration, cardiac pathology, inflammation and fibrosis, and frontotemporal dementia. Applications cited include WB, IF, IHC, FC, IP/CoIP, and electrophysiology.
| Species | Application | Title |
|---|---|---|
Nature A growth-factor-activated lysosomal K+ channel regulates Parkinson's pathology.
| ||
Nat Commun Deficiency of lysosomal TMEM175 in myeloid macrophages exerts anti-tumor immunity via inflammasome and cross-presentation pathway.
| ||
Mol Cell Lysosomal LAMP proteins regulate lysosomal pH by direct inhibition of the TMEM175 channel | ||
Adv Sci (Weinh) Trafficking Deficiency of TMEM175 Variants in Parkinson's Disease Pathogenesis and the Prospects of Precision Medicine. |
