TMEM176B Polyclonal antibody

TMEM176B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19825-1-AP

Synonyms

LR8, Protein LR8, TMEM176B, transmembrane protein 176B

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Validation Data Gallery

• 

  WB analysis of A549 using 19825-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19825-1-AP (TMEM176B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 19825-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19825-1-AP (TMEM176B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 19825-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19825-1-AP (TMEM176B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 19825-1-AP is TMEM176B Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM176B fusion protein Ag13918
• Full Name: transmembrane protein 176B
• Calculated Molecular Weight: 270 aa, 29 kDa
• Observed Molecular Weight: 31 kDa, 23 kDa
• GenBank Accession Number: BC004358
• Gene Symbol: TMEM176B
• Gene ID (NCBI): 28959
• RRID: AB_10638313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM176B (also known as LR8) is a transmembrane protein belongs to the MS4A family of proteins. It may play a role in the process of maturation of dendritic cells. TMEM176B was first discovered in human lung fibroblasts and is associated with human small cell lung carcinoma. Studies suggest that TMEM176B might be involved in cancer and might serve as targets for antiangiogenic therapy of cancer patients (PMID: 24602018, 22244448). There are two isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	IF	Int J Biol Macromol; Formulating a TMEM176B blocker in chitosan nanoparticles uncouples its paradoxical roles in innate and adaptive antitumoral immunity; Authors - Sabina Victoria
human	WB	Front Genet; Comprehensive Analysis of Expression and Prognostic Value of MS4As in Glioma.; Authors - Yingying Zeng
		Cancer Cell; Targeting TMEM176B Enhances Antitumor Immunity and Augments the Efficacy of Immune Checkpoint Blockers by Unleashing Inflammasome Activation.; Authors - Mercedes Segovia