TMEM181 Polyclonal antibody

TMEM181 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55411-1-AP

Synonyms

GPR178, KIAA1423, TMEM181, transmembrane protein 181

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Validation Data Gallery

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  WB analysis of A431 using 55411-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55411-1-AP (TMEM181 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 55411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55411-1-AP (TMEM181 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55411-1-AP targets TMEM181 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transmembrane protein 181
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: NM_020823
• Gene Symbol: TMEM181
• Gene ID (NCBI): 57583
• RRID: AB_11182284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM181, also named as GPR178, KIAA1423, is a membrane protein. It mediates action of cytolethal distending toxins (CDT), which are secreted by many pathogenic bacteria. Expression level of TMEM181 is rate-limiting for intoxication. This antibody is specific to TMEM181.