TMEM199 Polyclonal antibody

TMEM199 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25489-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

C17orf32, TMEM199, transmembrane protein 199

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of NIH/3T3 using 25489-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25489-1-AP (TMEM199 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 25489-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 25489-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 25489-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 25489-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	NIH/3T3 cells
Positive IHC detected in	human breast cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25489-1-AP targets TMEM199 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22009 Product name: Recombinant human TMEM199 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-208 aa of BC033113 Sequence: MASSLLAGERLVRALGPGGELEPERLPRKLRAELEAALGKKHKGGDSSSGPQRLVSFRLIRDLHQHLRERDSKLYLHELLEGSEIYLPEVVKPPRNPELVARLEKIKIQLANEEYKRITRNVTCQDTRHGGTLSDLGKQVRSLKALVITIFNFIVTVVAAFVCTYLGSQYIFTEMASRVLAALIVASVVGLAELYVMVRAMEGELGEL Predict reactive species
Full Name	transmembrane protein 199
Calculated Molecular Weight	208 aa, 23 kDa
Observed Molecular Weight	18-23 kDa
GenBank Accession Number	BC033113
Gene Symbol	TMEM199
Gene ID (NCBI)	147007
RRID	AB_2880101
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N511
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM199, also named as C17orf32, is a component of the complex sense antisense architecture (CSAGA).

Protocols

Product Specific Protocols
IHC protocol for TMEM199 antibody 25489-1-AP	Download protocol
WB protocol for TMEM199 antibody 25489-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TMEM199 Polyclonal antibody (Cat# 25489-1-AP) has 1 total citation. It appears in the journal Oncogene (Impact Factor 9.1), published in 2024. The cited study investigated how USP33 facilitates ovarian cancer progression by deubiquitinating and stabilizing CBX2, placing this citation within the research fields of cancer biology and protein ubiquitination/deubiquitination pathways.