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  • KD/KO Validated

TMEM41B Polyclonal antibody

TMEM41B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 29270-1-AP

Synonyms

KIAA0033, TMEM41B, transmembrane protein 41B



Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inmouse lung tissue, mouse testis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

29270-1-AP targets TMEM41B in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM41B fusion protein Ag30810
Full Name transmembrane protein 41B
Observed molecular weight22-28 kDa
GenBank accession numberBC035034
Gene symbol TMEM41B
Gene ID (NCBI) 440026
RRIDAB_2918264
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane protein 41B (TMEM41B), also known as Stasimon, is a major target of survival motor neuron (SMN)-dependent U12 splicing and has been identified as a protein required for motor circuit function (PMID: 23063131). TMEM41B localizes to the endoplasmic reticulum. It is involved in autophagosome biogenesis and lipid mobilization (PMID: 30126924). TMEM41B has been shown to be essential for mouse embryonic development (PMID: 30352685). Additionally, genome-scale CRISPR knockout screens reveal that TMEM41B is required for infection of flavivirus and coronavirus, including SARS-CoV-2 (PMID: 33052348; 33052332). Alternative splicing results in three transcript variants encoding distinct isoforms.

Protocols

Product Specific Protocols
WB protocol for TMEM41B antibody 29270-1-APDownload protocol
IHC protocol for TMEM41B antibody 29270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
  • KO Validated
mouseIHC

Biomedicines

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Authors - Xiuying Liang
humanWB

STAR Protoc

In vitro and in vivo assay of the ER lipid scramblase TMEM41B.

Authors - Lingzhi Wu
  • KO Validated
mouse,pigWB,IF

J Virol

TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication

Authors - Xiu-Qing Li
  • KD Validated
humanWB

Nature

PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection

Authors - Dijin Xu