Tested Applications
| Positive WB detected in | mouse brain tissue, mouse ovary tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17095-1-AP targets TMEM5 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10562 Product name: Recombinant human TMEM5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 96-425 aa of BC013152 Sequence: TDLSVQIWGKAAIGLYLWEHIFEGLLDPSDVTAQWREGKSIVGRTQYSFITGPAVIPGYFSVDVNNVVLILNGREKAKIFYATQWLLYAQNLVQIQKLQHLAVVLLGNEHCDNEWINPFLKRNGGFVELLFIIYDSPWINDVDVFQWPLGVATYRNFPVVEASWSMLHDERPYLCNFLGTIYENSSRQALMNILKKDGNDKLCWVSAREHWQPQETNESLKNYQDALLQSDLTLCPVGVNTECYRIYEACSYGSIPVVEDVMTAGNCGNTSVHHGAPLQLLKSMGAPFIFIKNWKELPAVLEKEKTIILQEKIERRKMLLQWYQHFKTEL Predict reactive species |
| Full Name | transmembrane protein 5 |
| Calculated Molecular Weight | 443 aa, 51 kDa |
| Observed Molecular Weight | 45-51 kDa |
| GenBank Accession Number | BC013152 |
| Gene Symbol | TMEM5 |
| Gene ID (NCBI) | 10329 |
| RRID | AB_2878349 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2B1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transmembrane protein 5 (TMEM5) is associated with gonadal dysgenesis and neural tube defects.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM5 antibody 17095-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TMEM5 Polyclonal antibody (Cat# 17095-1-AP) has 1 citation to date. It appears in the European Journal of Pharmaceutical Sciences (IF 5.1), in a study investigating TMEM5 as a therapeutic target in glioblastoma, where modulation of CLOCK and CRY1 expression via the circadian signaling axis was used to restore temozolomide sensitivity. The research fields associated with this citation include glioblastoma/cancer biology, circadian rhythm signaling, and drug resistance.
| Species | Application | Title |
|---|---|---|
Eur J Pharm Sci Targeting the xylosyltransferase TMEM5 in glioblastoma to modulate CLOCK and CRY1 expression and restore temozolomide sensitivity via the circadian signaling axis.
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