TMEM55B Polyclonal antibody

• Featured Product
• KD/KO Validated

TMEM55B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 23992-1-AP

Synonyms

C14orf9, PtdIns 4,5 P2 4 Ptase I, TMEM55B, transmembrane protein 55B

---

Validation Data Gallery

• 

  WB analysis using 23992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23992-1-AP (TMEM55B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23992-1-AP (TMEM55B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 23992-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23992-1-AP (TMEM55B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23992-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23992-1-AP (TMEM55B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23992-1-AP

  IP result of anti-TMEM55B (IP:23992-1-AP, 4ug; Detection:23992-1-AP 1:500) with HeLa cells lysate 1600 ug.

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, A549 cells, HepG2 cells, MCF-7 cells, rat heart tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Product Information

The immunogen of 23992-1-AP is TMEM55B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM55B fusion protein Ag21172
• Full Name: transmembrane protein 55B
• Calculated Molecular Weight: 277 aa, 29 kDa
• Observed Molecular Weight: 29-32 kDa
• GenBank Accession Number: BC002867
• Gene Symbol: TMEM55B
• Gene ID (NCBI): 90809
• RRID: AB_2879391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM55B is also named as C14orf9 and PIP4P1. TMEM55B is originally identified as phosphatidylinositol-4,5-P24-phosphatases (PtdIns-4,5-P24-phosphatases) that catalyze dephosphorylation of PtdIns-4,5-P2 to PtdIns-5-P. The transmembrane protein TMEM55B is involved in the perinuclear clustering of lysosomes upon starvation. TMEM55B expression is upregulated upon starvation via TFEB and TFE3 activation, and loss of TMEM55B diminishes starvation-induced lysosomal clustering. TMEM55B can bind to the dynein adaptor JIP4, promoting dynein-dynactin-dependent lysosomal trafficking to the perinuclear region. TMEM55B is also suggested to contribute to lysosomal homeostasis and mTORC1 activation via the V-ATPase assembly (PMID: 33537719).

Publications

Species	Application	Title
rat	WB	Nat Commun; Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation.; Authors - Michiko Shirane ; KD Validated
mouse	WB	JCI Insight; TFEB-mediated lysosomal exocytosis alleviates high fat diet-induced lipotoxicity in the kidney; Authors - Jun Nakamura
mouse	WB,IP	Arterioscler Thromb Vasc Biol; Phosphatidylinositol-(4,5)-Bisphosphate Regulates Plasma Cholesterol Through LDL (Low-Density Lipoprotein) Receptor Lysosomal Degradation.; Authors - Yuanyuan Qin ; KD Validated
human	WB	Genes Cells; TMEM55B contributes to lysosomal homeostasis and amino acid-induced mTORC1 activation.; Authors - Yutaka Hashimoto ; KD Validated
monkey	WB	J Cell Biol; Tex2 is required for lysosomal functions at TMEM55-dependent ER membrane contact sites; Authors - Yuanjiao Du ; KD Validated
human	WB,IP	Nat Commun; TMEM55B links autophagy flux, lysosomal repair, and TFE3 activation in response to oxidative stress; Authors - Eutteum Jeong