TMEM64 Polyclonal antibody

TMEM64 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21274-1-AP

Synonyms

TMEM64, transmembrane protein 64

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Validation Data Gallery

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  WB analysis using 21274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21274-1-AP (TMEM64 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 21274-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

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  IHC staining of human prostate cancer using 21274-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21274-1-AP targets TMEM64 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM64 fusion protein Ag15753
• Full Name: transmembrane protein 64
• Calculated Molecular Weight: 380 aa, 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC113828
• Gene Symbol: TMEM64
• Gene ID (NCBI): 169200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tmem64 (transmembrane protein 64) is a positive modulator of osteoclast differentiation by SERCA2-dependent Ca2+ signaling (PMID: 23395171). Altered Levels of mRNAs for Calcium-Binding/Associated Proteins, Annexin A1, S100A4, and TMEM64 are associated with osteoporosis in peripheral blood mononuclear cells (PMID: 31814858). Tmem64 is involved in the metastatic progression of prostate cancer cells by regulating Wnt3a secretion (PMID: 31289498).