TMEM65 Polyclonal antibody

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  WB analysis using 21913-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21913-1-AP

  WB result of TMEM65 antibody (21913-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HEK-293 cells.

• 

  WB analysis using 21913-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of Huh-7 using 21913-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Huh-7 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

TMEM65 Polyclonal Antibody for WB, ELISA

Cat No. 21913-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Transmembrane protein 65

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, HeLa cells, Neuro-2a cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21913-1-AP targets TMEM65 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15509

  Product name: Recombinant human TMEM65 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-178 aa of BC032396

  Sequence: MEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEAPPPTPGQLRYVFIHNAIPFIGFGFLDNAIMIVAGTHIEMSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRLSTHLGKAVGVTIGCILGMFPLIFFGGGEEDEKLETKS

• Full Name: transmembrane protein 65
• Calculated Molecular Weight: 240 aa, 26 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC032396
• Gene Symbol: TMEM65
• Gene ID (NCBI): 157378
• RRID: AB_2918075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6PI78
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM65 (Transmembrane protein 65) is an inner-mitochondrial-membrane protein essential for calcium homeostasis and organelle integrity. By binding to and stabilizing the Na⁺/Ca²⁺ exchanger NCLX, it facilitates mitochondrial Ca²⁺ efflux, thereby regulating oxidative phosphorylation and limiting ROS production. In cardiomyocytes, TMEM65 localizes to intercalated discs where it interacts with connexin-43, ensuring normal electrical conduction and preventing arrhythmia. Loss-of-function mutations cause mitochondrial myopathy with neurodevelopmental delay, whereas its over-expression in gastric cancer activates the YWHAZ-PI3K-Akt-mTOR axis, promoting tumorigenesis. Thus, TMEM65 links mitochondrial quality control to cytoplasmic signaling, positioning it as a potential therapeutic target in metabolic and oncologic diseases.