TMEM65 Recombinant monoclonal antibody

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  WB analysis using 83219-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83219-4-RR (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83219-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83219-4-RR (TMEM65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83219-4-RR

  WB result of TMEM65 antibody (83219-4-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 83219-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83219-4-RR against Human TMEM65 were performed. The affinity constant is 0.365 nM.

• Featured Product
• KD/KO Validated

TMEM65 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83219-4-RR

Clone No.230512D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

230512D7, Transmembrane protein 65

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, mouse brain tissue, PC-3 cells, SH-SY5Y cells, HeLa cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83219-4-RR targets TMEM65 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15509

  Product name: Recombinant human TMEM65 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-178 aa of BC032396

  Sequence: MEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEAPPPTPGQLRYVFIHNAIPFIGFGFLDNAIMIVAGTHIEMSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRLSTHLGKAVGVTIGCILGMFPLIFFGGGEEDEKLETKS

• Full Name: transmembrane protein 65
• Calculated Molecular Weight: 240 aa, 26 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC032396
• Gene Symbol: TMEM65
• Gene ID (NCBI): 157378
• RRID: AB_3670904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q6PI78
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM65 (Transmembrane protein 65) is an inner-mitochondrial-membrane protein essential for calcium homeostasis and organelle integrity. By binding to and stabilizing the Na⁺/Ca²⁺ exchanger NCLX, it facilitates mitochondrial Ca²⁺ efflux, thereby regulating oxidative phosphorylation and limiting ROS production. In cardiomyocytes, TMEM65 localizes to intercalated discs where it interacts with connexin-43, ensuring normal electrical conduction and preventing arrhythmia. Loss-of-function mutations cause mitochondrial myopathy with neurodevelopmental delay, whereas its over-expression in gastric cancer activates the YWHAZ-PI3K-Akt-mTOR axis, promoting tumorigenesis. Thus, TMEM65 links mitochondrial quality control to cytoplasmic signaling, positioning it as a potential therapeutic target in metabolic and oncologic diseases.