TMEM98 Polyclonal antibody

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  WB analysis of mouse E12.5 embryos using 14731-1-AP

  WB result of anti-TMEM98(14731-1-AP) in WT and KO mouse (10 ug of total protein lysate from E12.5 embryos) by Dr. Sally H. Cross.

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  IP experiment of Y79 using 14731-1-AP

  IP result of anti-TMEM98 (IP:14731-1-AP, 4ug; Detection:14731-1-AP 1:300) with Y79 cells lysate 2400 ug.

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  IHC staining of mouse embryo using 14731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14731-1-AP (TMEM98 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

TMEM98 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14731-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Protein TADA1, TMEM98, transmembrane protein 98

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Tested Applications

• Positive WB detected in: mouse E12.5 embryos
• Positive IP detected in: Y79 cells
• Positive IHC detected in: mouse embryo tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Product Information

14731-1-AP targets TMEM98 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6492

  Product name: Recombinant human TMEM98 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-226 aa of BC000526

  Sequence: CRQRYCRPRDLLQRYDSKPIVDLIGAMETQSEPSELELDDVVITNPHIEAILENEDWIEDASGLMSHCIAILKICHTLTEKLVAMTMGSGAKMKTSASVSDIIVVAKRISPRVDDVVKSMYPPLDPKLLDARTTALLLSVSHLVLVTRNACHLTGGLDWIDQSLSAAEEHLEVLREAALASEPDKGLPGPEGFLQEQSAI

• Full Name: transmembrane protein 98
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: BC000526
• Gene Symbol: TMEM98
• Gene ID (NCBI): 26022
• RRID: AB_11182589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2Y6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM98, also named as TADA1, composed of 226 amino acid residues, is a potential single-pass transmembrane protein. It is expression in both nucleus and cytoplasm. TMEM98 was found to be over-expressed in most of the hepatocellular carcinoma tissue. MEM98 is not only a prognostic marker for patients with hepatocellular carcinoma but also a novel molecular target associated with intrinsic and acquired chemoresistance of hepatocellular carcinoma (PMID: 24608572).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	PLoS Genet; Variants in myelin regulatory factor (MYRF) cause autosomal dominant and syndromic nanophthalmos in humans and retinal degeneration in mice.; Authors - Sarah J Garnai
mouse	WB,IF	PLoS Genet; The nanophthalmos protein TMEM98 inhibits MYRF self-cleavage and is required for eye size specification.; Authors - Sally H Cross
human	WB	Aging (Albany NY); The microRNA miR-29c-5p inhibits cell proliferation and migration by targeting TMEM98 in head and neck carcinoma.; Authors - Jingjia Li
mouse	IF	Br J Ophthalmol; Clinical features of patients with mutations in genes for nanophthalmos; Authors - Xueqing Li
mouse	WB,IF	Invest Ophthalmol Vis Sci; Missense Mutations in the Human Nanophthalmos Gene TMEM98 Cause Retinal Defects in the Mouse.; Authors - Sally H Cross ; KO Validated
human	WB,CoIP	PLoS One; TMEM98 is a negative regulator of FRAT mediated Wnt/ß-catenin signalling.; Authors - Tanne van der Wal