TMEM98 Recombinant monoclonal antibody

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  WB analysis of HepG2 using 84284-5-RR

  WB result of TMEM98 antibody (84284-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM98 transfected HepG2 cells.

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  WB analysis using 84284-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84284-5-RR (TMEM98 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84284-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84284-5-RR against Human TMEM98 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

TMEM98 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84284-5-RR

Clone No.241581A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

241581A6, Protein TADA1, Transmembrane protein 98, UNQ536/PRO1079

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, Y79 cells, NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84284-5-RR targets TMEM98 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6492

  Product name: Recombinant human TMEM98 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-226 aa of BC000526

  Sequence: CRQRYCRPRDLLQRYDSKPIVDLIGAMETQSEPSELELDDVVITNPHIEAILENEDWIEDASGLMSHCIAILKICHTLTEKLVAMTMGSGAKMKTSASVSDIIVVAKRISPRVDDVVKSMYPPLDPKLLDARTTALLLSVSHLVLVTRNACHLTGGLDWIDQSLSAAEEHLEVLREAALASEPDKGLPGPEGFLQEQSAI

• Full Name: transmembrane protein 98
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000526
• Gene Symbol: TMEM98
• Gene ID (NCBI): 26022
• RRID: AB_3671831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y2Y6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM98, also named as TADA1, composed of 226 amino acid residues, is a potential single-pass transmembrane protein. It is expression in both nucleus and cytoplasm. TMEM98 was found to be over-expressed in most of the hepatocellular carcinoma tissue. MEM98 is not only a prognostic marker for patients with hepatocellular carcinoma but also a novel molecular target associated with intrinsic and acquired chemoresistance of hepatocellular carcinoma (PMID: 24608572).