TMF1 Polyclonal antibody

TMF1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 22212-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Androgen receptor-associated protein of 160 kDa, ARA160, TATA element modulatory factor, TMF

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis of mouse brain using 22212-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 22212-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat brain using 22212-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse colon using 22212-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 22212-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse brain using 22212-1-AP
IP result of anti-TMF1 (IP:22212-1-AP, 4ug; Detection:22212-1-AP 1:5000) with mouse brain tissue lysate 2100 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 22212-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 22212-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 22212-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22212-1-AP (TMF1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 22212-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 22212-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, Jurkat cells, mouse colon tissue, rat brain tissue, human brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human colon cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22212-1-AP targets TMF1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17537 Product name: Recombinant human TMF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 783-1093 aa of BC126123 Sequence: GSQTSSWEKLEKNLSDRLGESQTLLAAAVERERAATEELLANKIQMSSMESQNSLLRQENSRFQAQLESEKNRLCKLEDENNRYQVELENLKDEYVRTLEETRKEKTLLNSQLEMERMKVEQERKKAIFTQETIKEKERKPFSVSSTPTMSRSSSISGVDMAGLQTSFLSQDESHDHSFGPMPISANGSNLYDAVRMGAGSSIIENLQSQLKLREGEITHLQLEIGNLEKTRSIMAEELVKLTNQNDELEEKVKEIPKLRTQLRDLDQRYNTILQMYGEKAEEAEELRLDLEDVKNMYKTQIDELLRQSLS Predict reactive species
Full Name	TATA element modulatory factor 1
Calculated Molecular Weight	1093 aa, 123 kDa
Observed Molecular Weight	150-160 kDa
GenBank Accession Number	BC126123
Gene Symbol	TMF1
Gene ID (NCBI)	7110
RRID	AB_2879031
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P82094
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMF1, also named as TMF and ARA160, is a potential coactivator of the androgen receptor. It mediates STAT3 degradation. The MW of TMF1 is about 160 kDa.

Protocols

Product Specific Protocols
IHC protocol for TMF1 antibody 22212-1-AP	Download protocol
IP protocol for TMF1 antibody 22212-1-AP	Download protocol
WB protocol for TMF1 antibody 22212-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TMF1 Polyclonal antibody (Cat# 22212-1-AP) has 2 citations. One appears in Ecotoxicology and Environmental Safety, studying PFOA exposure effects on sperm head shaping in mice. The other appears in Neurobiology of Disease, investigating how RAB3GAP1 modulates neuronal morphogenesis and interacts with axon elongation and ER-Golgi trafficking factors in human cells. The associated research fields are reproductive toxicology and neuroscience/neurodevelopment.

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. Authors - Chuan Wang View Article
human	WB,IP,IF,CoIP	Neurobiol Dis The Warburg micro syndrome protein RAB3GAP1 modulates neuronal morphogenesis and interacts with axon elongation end ER-Golgi trafficking factors Authors - Pankaj S Ghate View Article