TMF1 Polyclonal antibody

TMF1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22212-1-AP

Synonyms

TMF, TATA element modulatory factor, ARA160, Androgen receptor-associated protein of 160 kDa

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Validation Data Gallery

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  WB analysis of mouse brain using 22212-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 22212-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22212-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 22212-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22212-1-AP (TMF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 22212-1-AP

  IP result of anti-TMF1 (IP:22212-1-AP, 4ug; Detection:22212-1-AP 1:5000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of human colon cancer using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22212-1-AP (TMF1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22212-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22212-1-AP (TMF1 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse brain tissue, Jurkat cells, mouse colon tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

22212-1-AP targets TMF1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMF1 fusion protein Ag17537
• Full Name: TATA element modulatory factor 1
• Calculated Molecular Weight: 1093 aa, 123 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC126123
• Gene Symbol: TMF1
• Gene ID (NCBI): 7110
• RRID: AB_2879031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMF1, also named as TMF and ARA160, is a potential coactivator of the androgen receptor. It mediates STAT3 degradation. The MW of TMF1 is about 160 kDa.

Publications

Species	Application	Title
human	WB,IF,IP,CoIP	Neurobiol Dis; The Warburg micro syndrome protein RAB3GAP1 modulates neuronal morphogenesis and interacts with axon elongation end ER-Golgi trafficking factors; Authors - Pankaj S Ghate