TMF1 Monoclonal antibody, PBS Only

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  WB analysis using 67505-1-Ig (same clone as 67505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67505-1-Ig (TMF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67505-1-Ig (same clone as 67505-1-PBS)

  WB result of TMF1 antibody (67505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMF1 transfected HeLa cells. This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TMF1 antibody (67505-1-Ig, Clone: 3G12F2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMF1 antibody (67505-1-Ig, Clone: 3G12F2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TMF1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67505-1-PBS

Clone No.3G12F2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Androgen receptor-associated protein of 160 kDa, ARA160, TATA element modulatory factor, TMF

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Product Information

67505-1-PBS targets TMF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17666

  Product name: Recombinant human TMF1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 783-1093 aa of BC126123

  Sequence: GSQTSSWEKLEKNLSDRLGESQTLLAAAVERERAATEELLANKIQMSSMESQNSLLRQENSRFQAQLESEKNRLCKLEDENNRYQVELENLKDEYVRTLEETRKEKTLLNSQLEMERMKVEQERKKAIFTQETIKEKERKPFSVSSTPTMSRSSSISGVDMAGLQTSFLSQDESHDHSFGPMPISANGSNLYDAVRMGAGSSIIENLQSQLKLREGEITHLQLEIGNLEKTRSIMAEELVKLTNQNDELEEKVKEIPKLRTQLRDLDQRYNTILQMYGEKAEEAEELRLDLEDVKNMYKTQIDELLRQSLS

• Full Name: TATA element modulatory factor 1
• Calculated Molecular Weight: 1093 aa, 123 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC126123
• Gene Symbol: TMF1
• Gene ID (NCBI): 7110
• RRID: AB_2882728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P82094
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.