TMOD1 Polyclonal antibody

TMOD1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10145-1-AP

Synonyms

D9S57E, E Tmod, Erythrocyte tropomodulin, ETMOD, TMOD, TMOD1, tropomodulin 1

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Validation Data Gallery

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  WB analysis using 10145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10145-1-AP (TMOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 10145-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10145-1-AP (TMOD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 10145-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10145-1-AP (TMOD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 10145-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using TMOD1 antibody (10145-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 10145-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using TMOD1 antibody (10145-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart, mouse skeletal muscle, rat brain
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

10145-1-AP targets TMOD1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMOD1 fusion protein Ag0192
• Full Name: tropomodulin 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC002660
• Gene Symbol: TMOD1
• Gene ID (NCBI): 7111
• RRID: AB_2205316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actin filaments control cell morphology. The length of these filaments is regulated in muscle and nonmuscle cell types by tropomodulins 1-4 (Tmod1-4), a family of proteins that cap the pointed ends of actin filaments. The molecular weight of Tmod 1 is about 40 kDa.

Publications

Species	Application	Title
mouse	WB	Glia; S1P1 deletion in oligodendroglial lineage cells: Effect on differentiation and myelination.; Authors - Danuta E Dukala
human,mouse,rat	WB	Commun Biol; Recessive TMOD1 mutation causes childhood cardiomyopathy; Authors - Catalina Vasilescu
human	IHC	Am J Surg Pathol; Identification of a unique gene expression signature that differentiates hepatocellular adenoma from well-differentiated hepatocellular carcinoma.; Authors - Chen Zong-Ming E ZM
mouse	IF	J Biol Chem; The actin-organizing formin protein Fhod3 is required for postnatal development and functional maintenance of the adult heart in mice.; Authors - Tomoki Ushijima
mouse	IHC	PLoS One; Expression and subcellular localization of mammalian formin Fhod3 in the embryonic and adult heart.; Authors - Kan-o Meikun M
mouse	IF	PLoS One; Transgenic Expression of the Formin Protein Fhod3 Selectively in the Embryonic Heart: Role of Actin-Binding Activity of Fhod3 and Its Sarcomeric Localization during Myofibrillogenesis.; Authors - Noriko Fujimoto