TMOD2 Polyclonal antibody

TMOD2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15262-1-AP

Synonyms

N TMOD, Neuronal tropomodulin, NTMOD, TMOD2, Tropomodulin 2, tropomodulin 2 (neuronal)

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Validation Data Gallery

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  WB analysis of human testis using 15262-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15262-1-AP (TMOD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15262-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15262-1-AP (TMOD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 15262-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15262-1-AP (TMOD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 15262-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15262-1-AP (TMOD2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse testis using 15262-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15262-1-AP (TMOD2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 15262-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15262-1-AP (TMOD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 15262-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 15262-1-AP (TMOD2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 15262-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 15262-1-AP (TMOD2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human testis tissue, human brain tissue, human colon tissue, mouse brain tissue, mouse colon tissue, mouse testis tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15262-1-AP targets TMOD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMOD2 fusion protein Ag7262
• Full Name: tropomodulin 2 (neuronal)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC064961
• Gene Symbol: TMOD2
• Gene ID (NCBI): 29767
• RRID: AB_2256189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Biol Chem; Transforming growth factor beta 1 alters the 3'UTR of mRNA to promote lung fibrosis.; Authors - Junsuk Ko