TMPO/LAP2 Monoclonal antibody, PBS Only

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  WB analysis using 67157-1-Ig (same clone as 67157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 67157-1-Ig (same clone as 67157-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67157-1-Ig (same clone as 67157-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67157-1-Ig (same clone as 67157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67157-1-Ig (same clone as 67157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67157-1-Ig (same clone as 67157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67157-1-Ig (same clone as 67157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67157-1-Ig (same clone as 67157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67157-1-Ig (same clone as 67157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67157-1-Ig (same clone as 67157-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (67157-1-Ig, Clone: 3G4E1 ) at dilution of 1:2200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

TMPO/LAP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67157-1-PBS

Clone No.3G4E1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LAP2, TMPO, 3G4E1, Lamina-associated polypeptide 2, isoforms beta/gamma, Thymopentin

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Product Information

67157-1-PBS targets TMPO/LAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6415

  Product name: Recombinant human TMPO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-362 aa of BC053675

  Sequence: MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFPYEASTPTGISAS

• Full Name: thymopoietin
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa, 39 kDa
• GenBank Accession Number: BC053675
• Gene Symbol: TMPO/LAP2
• Gene ID (NCBI): 7112
• RRID: AB_2882454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P42167
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).