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TMPRSS4 Polyclonal antibody

TMPRSS4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11283-1-AP

Synonyms

CAPH2, Channel activating protease 2, MT SP2, TMPRSS3, TMPRSS4



Tested Applications

Positive WB detected inSW 1990 cells, BxPC-3 cells, mouse stomach tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue, human colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11283-1-AP targets TMPRSS4 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMPRSS4 fusion protein Ag1804
Full Name transmembrane protease, serine 4
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC011703
Gene Symbol TMPRSS4
Gene ID (NCBI) 56649
RRIDAB_2203508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMPRSS4, also named as CAPH2 and MT-SP2, belongs to the peptidase S1 family. TMPRSS4 is a novel type II transmembrane serine protease that is highly expressed on the cell surface in pancreatic, thyroid and other cancer tissues. It promotes invasion, migration and metastasis of human tumor cells by facilitating an epithelial-mesenchymal transition(EMT)(PMID:20118200).

Protocols

Product Specific Protocols
WB protocol for TMPRSS4 antibody 11283-1-APDownload protocol
IHC protocol for TMPRSS4 antibody 11283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gastroenterology

Angiotensin-converting Enzyme 2-containing Extracellular Vesicles and Exomeres Bind the Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein.

Authors - Qin Zhang
humanWB

Cancer Res

TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation.

Authors - David J Drasin
mouseWB

Int J Mol Sci

Cardiac and Renal SARS-CoV-2 Viral Entry Protein Regulation by Androgens and Diet: Implications for Polycystic Ovary Syndrome and COVID-19.

Authors - Samar Rezq
humanWB,IHC,IF

Carcinogenesis

TMPRSS4 induces invasion and epithelial-mesenchymal transition through upregulation of integrin alpha5 and its signaling pathways.

Authors - Kim Semi S
human, mouseWB, IHC, IF

Cancer Lett

TMPRSS4 induces cancer stem cell-like properties in lung cancer cells and correlates with ALDH expression in NSCLC patients.

Authors - Arrate L de Aberasturi
humanIHC

Front Immunol

TSPAN1, TMPRSS4, SDR16C5, and CTSE as Novel Panel for Pancreatic Cancer: A Bioinformatics Analysis and Experiments Validation.

Authors - Hua Ye