TMPRSS6 Polyclonal antibody

TMPRSS6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12950-1-AP

Synonyms

IRIDA, Matriptase 2, TMPRSS6

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 12950-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12950-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12950-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12950-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12950-1-AP (TMPRSS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, MCF-7 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

12950-1-AP targets TMPRSS6 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMPRSS6 fusion protein Ag4076
• Full Name: transmembrane protease, serine 6
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC039082
• Gene Symbol: TMPRSS6
• Gene ID (NCBI): 164656
• RRID: AB_2877896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The type II transmembrane serine protease TMPRSS6 (also known as matriptase-2) controls iron homeostasis through its negative regulation of expression of hepcidin, a key hormone involved in iron metabolism. Upstream of the hepcidin-regulated signaling pathway, TMPRSS6 cleaves its target substrate hemojuvelin (HJV) at the plasma membrane(PMID:21724843).It Belongs to the peptidase S1 family.Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA).It has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Haematologica; New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia.; Authors - Jing Liu
human,mouse	WB,IF	Cell Death Dis; Transmembrane serine protease 6, a novel target for inhibition of neuronal tumor growth; Authors - Yong Zuo ; KD Validated