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- KD/KO Validated
Thymosin beta 4 Polyclonal antibody
Thymosin beta 4 Polyclonal Antibody for IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 19850-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
| IHC | See 6 publications below |
| IF | See 3 publications below |
Product Information
19850-1-AP targets Thymosin beta 4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Thymosin beta 4 fusion protein Ag13914 |
| Full Name | thymosin beta 4, X-linked |
| Calculated Molecular Weight | 63 aa, 7 kDa |
| Observed Molecular Weight | 5 kDa |
| GenBank Accession Number | BC001631 |
| Gene Symbol | Thymosin beta 4 |
| Gene ID (NCBI) | 7114 |
| RRID | AB_10642437 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Thymosin beta 4 (Tβ4), encoded by TMSB4X gene, is an actin sequestering protein which plays an important role in the organization of the cytoskeleton. Numerous studies have demonstrated the effects of Tβ4 on cell migration, proliferation, apoptosis and inflammation after exogenous treatment (PMID: 22652458). Recently, novel findings provided compelling evidence that Tβ4 played a key role in facilitating tumor metastasis and angiogenesis. It has been found that Tβ4 expressed increasingly in a number of metastatic tumors, thus providing a potential target of opportunity for cancer management, especially for cancer metastasis therapy (PMID: 22856429).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Thymosin beta 4 antibody 19850-1-AP | Download protocol |
| IF protocol for Thymosin beta 4 antibody 19850-1-AP | Download protocol |
| IP protocol for Thymosin beta 4 antibody 19850-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Single-cell transcriptomic analysis of the tumor ecosystems underlying initiation and progression of papillary thyroid carcinoma | ||
J Mol Cell Biol Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3.
| ||
Haematologica Thymosin β4 is essential for thrombus formation by controlling the G-actin/F-actin equilibrium in platelets.
| ||
Cancer Gene Ther Lysine-40 succinylation of TAGLN2 induces glioma angiogenesis and tumor growth through regulating TMSB4X | ||
Sci Rep Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma. |
