TMUB1 Polyclonal antibody

TMUB1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16638-1-AP

Synonyms

C7orf21, DULP, HOPS, SB144, TMUB1, Ubiquitin like protein DULP, Ubiquitin like protein SB144, UNQ763/PRO1555

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Validation Data Gallery

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  WB analysis using 16638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16638-1-AP (TMUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 16638-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed L02 cells using TMUB1 antibody (16638-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HuH-7 cells, L02 cells
• Positive IF detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16638-1-AP targets TMUB1 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMUB1 fusion protein Ag9988
• Full Name: transmembrane and ubiquitin-like domain containing 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 27 kDa, 24 kDa
• GenBank Accession Number: BC000936
• Gene Symbol: TMUB1
• Gene ID (NCBI): 83590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1), also referred to as hepatocyte odd protein shuttling (HOPS), is a transmembrane ubiquitin-like protein that was originally identified in the regenerated liver. HOPS is a transmembrane protein released into the cell by an enzymatic cleavage. This allows HOPS to be shuttled from the nucleus to the cytoplasm and vice versa, depending on cell cycle and environmental stress conditions. HOPS cDNA expression gives rise to the translation of three different proteins with distinct molecular weights of 27, 24 and 21 kDa, respectively.The three isoforms of HOPS are differentially expressed depending on the tissue being examined. In the brain, the shortest isoform prevails over the other, whereas in the intestine the higher molecular weight isoform prevails among the other two. Indeed, HOPS shows a different concentration of the three isoforms, which can play different cell-dependent functions. The ratio between different isoforms may vary owing to physiopathological changes in the cell (PMID:32860479).16638-1-AP can detect two bands and some literature also detected two bands (PMID:30610893, 29777440, 29967478)