TMX4 Polyclonal antibody

TMX4 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21348-1-AP

Synonyms

KIAA1162, PDIA14, TMX4, TXNDC13, UNQ475/PRO938

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Validation Data Gallery

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  WB analysis of mouse brain using 21348-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21348-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21348-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 21348-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21348-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 21348-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21348-1-AP

  Immunofluorescent analysis of HepG2 cells using 21348-1-AP (TMX4 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 21348-1-AP

  1X10^6 HepG2 cells were stained with .2ug TMX4 antibody (21348-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: mouse brain tissue, A375 cells, human heart tissue, L02 cells
• Positive IHC detected in: human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21348-1-AP is TMX4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMX4 fusion protein Ag15696
• Full Name: thioredoxin-related transmembrane protein 4
• Calculated Molecular Weight: 349 aa, 39 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC033787
• Gene Symbol: TMX4
• Gene ID (NCBI): 56255
• RRID: AB_10733353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMX4(Thioredoxin-related transmembrane protein 4) is also named as KIAA1162, TXNDC13. It is a type I transmembrane protein, with a Trx-like domain facing the ER lumen and may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER. It could work as a reductase in the environment of the ER(PMID: 20056998). The full length protein has a 23 amino acid signal peptide. This antibody is specific to TMX4.

Publications

Species	Application	Title
	WB,IF	Nat Commun; TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress; Authors - Marika K Kucińska
human	WB	J Proteome Res; Mapping of the N-linked glycoproteome of human spermatozoa.; Authors - Wang Gaigai G