TNF-alpha Polyclonal antibody

TNF-alpha Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29652-1-AP

Synonyms

RATTNF, Tnf, TNF alpha, Tnfa

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Validation Data Gallery

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  WB analysis using 29652-1-AP

  PMA and ionomycin treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 29652-1-AP (TNF-alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29652-1-AP targets TNF-alpha in WB, ELISA applications and shows reactivity with rat, mouse samples.

• Tested Reactivity: rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNF-alpha fusion protein Ag31037
• Full Name: tumor necrosis factor (TNF superfamily, member 2)
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC107671
• Gene Symbol: Tnf
• Gene ID (NCBI): 24835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.