TSG-6 Polyclonal antibody

• Featured Product
• KD/KO Validated

TSG-6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13321-1-AP

Synonyms

Hyaluronate binding protein, TNF alpha induced protein 6, TNF stimulated gene 6 protein, TNFAIP6, TSG 6, TSG6, TSG-6

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 13321-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13321-1-AP (TSG-6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13321-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13321-1-AP (TSG-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, A549 cells
• Positive IHC detected in: rat kidney tissue, human cervical cancer tissue, human ovary tumor tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

13321-1-AP targets TSG-6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSG-6 fusion protein Ag3885
• Full Name: tumor necrosis factor, alpha-induced protein 6
• Calculated Molecular Weight: 277 aa, 31 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC030205
• Gene Symbol: TNFAIP6
• Gene ID (NCBI): 7130
• RRID: AB_2204641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	JACC Basic Transl Sci; Atheroprotective Effects of Tumor Necrosis Factor-Stimulated Gene-6.; Authors - Rena Watanabe
mouse	WB	Front Pharmacol; TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis via Suppression of STAT3 Activation.; Authors - Yue-Meng Wan
rat	WB	Neurochem Res; Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage.; Authors - Bin Tang
human	WB,IHC	Contrast Media Mol Imaging; TNFAIP6 Promotes Gastric Carcinoma Cell Invasion via Upregulating PTX3 and Activating the Wnt/β-Catenin Signaling Pathway; Authors - Hongquan Cui ; KD Validated
human	WB,IHC,IF	Neural Regen Res; Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis; Authors - Mingxiang Ding
human	WB,IHC	Front Immunol; Neutrophil infiltration associated genes on the prognosis and tumor immune microenvironment of lung adenocarcinoma; Authors - Renwang Liu