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  • KD/KO Validated

TNFAIP8 Polyclonal antibody

TNFAIP8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15790-1-AP

Synonyms

GG2 1, MDC 3.13, NDED, SCC S2, SCCS2, TNF alpha induced protein 8, TNF induced protein GG2 1, TNFAIP8



Tested Applications

Positive WB detected inK-562 cells, PC-13 cells, A549 cells, MOLT-4 cells
Positive IHC detected inhuman lung cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15790-1-AP targets TNFAIP8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFAIP8 fusion protein Ag8491
Full Name tumor necrosis factor, alpha-induced protein 8
Calculated Molecular Weight 198 aa, 23 kDa
Observed Molecular Weight 19-23 kDa
GenBank Accession NumberBC007014
Gene Symbol TNFAIP8
Gene ID (NCBI) 25816
RRIDAB_10792805
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFAIP8 (tumor necrosis factor, alpha-induced protein 8), also called SCC-S2/GG2-1/NDED, is associated with enhanced cell survival and inhibition of apoptosis. The induction of TNFAIP8 by TNF depends on the activation of NFκB. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. TNFAIP8 is expressed in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney at high levels, also expressed in various tumor tissues and all cancer cell lines tested. There are four major isoforms of TNFAIP8.

Protocols

Product Specific Protocols
WB protocol for TNFAIP8 antibody 15790-1-APDownload protocol
IHC protocol for TNFAIP8 antibody 15790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Immunol

Crucial Roles of TNFAIP8 Protein in Regulating Apoptosis and Listeria Infection.

Authors - Thomas P Porturas
humanWB

Oncotarget

TNFAIP8 promotes prostate cancer cell survival by inducing autophagy.

Authors - Suryakant Niture
  • KD Validated
humanWB

Toxins (Basel)

Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis

Authors - Suryakant Niture
humanIHC

J Proteome Res

Proteomic Analysis of Plasma sEVs Reveals That TNFAIP8 Is a New Biomarker of Cell Proliferation in Diabetic Retinopathy.

Authors - Jing Xiao
  • KD Validated
mouseIF

Sci Rep

The TIPE Molecular Pilot That Directs Lymphocyte Migration in Health and Inflammation.

Authors - Honghong Sun
  • KO Validated
mouseWB

Sci Rep

The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin.

Authors - Ji-Soo Kim
  • KD Validated