TNFRSF13B/CD267 Polyclonal antibody

TNFRSF13B/CD267 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21520-1-AP

Synonyms

CD267, TNFRSF13B, TACI, CVID

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Validation Data Gallery

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  WB analysis using 21520-1-AP

  IM-9 cells were subjected to SDS PAGE followed by western blot with 21520-1-AP (TNFRSF13B/CD267 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21520-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21520-1-AP (TNFRSF13B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: IM-9 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21520-1-AP targets TNFRSF13B/CD267 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFRSF13B/CD267 fusion protein Ag16097
• Full Name: tumor necrosis factor receptor superfamily, member 13B
• Calculated Molecular Weight: 293 aa, 32 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC109392
• Gene Symbol: TNFRSF13B/CD267
• Gene ID (NCBI): 23495
• RRID: AB_10888631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B), also known as CD267 or TACI, is a transmembrane protein of the TNF receptor superfamily found predominantly on B cells (PMID: 10920230). TACI is also expressed on activated T cells, myeloma cells, and expressed by monocytes intracellularly at a low level (PMID: 11429548; 16825497). TNFRSF13B is a receptor for TNFSF13/APRIL/CD256 and TNFSF13B/BAFF/CD257 and mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-κB and AP-1 (PMID: 10956646; 10973284; 9311921). It is involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.