DR6 Monoclonal antibody

DR6 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C2D10

Cat no : 66754-1-Ig

Synonyms

BM 018, CD358, Death receptor 6, DR6, TNFRSF21



Tested Applications

Positive WB detected inK-562 cells, THP-1 cells, Jurkat cells, Raji cells, Daudi cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66754-1-Ig targets DR6 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DR6 fusion protein Ag14327
Full Name tumor necrosis factor receptor superfamily, member 21
Calculated Molecular Weight 655 aa, 72 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC021572
Gene Symbol TNFRSF21
Gene ID (NCBI) 27242
RRIDAB_2882100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DR6 (also known as TNFRSF21 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).

Protocols

Product Specific Protocols
WB protocol for DR6 antibody 66754-1-IgDownload protocol
IF protocol for DR6 antibody 66754-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols