DR3 Polyclonal antibody

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  WB analysis of PC-3 using 55397-1-AP

  WB result of DR3 antibody (55397-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DR3 transfected PC-3 cells.

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  WB analysis using 55397-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55397-1-AP (DR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 55397-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55397-1-AP (DR3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 55397-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55397-1-AP (DR3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

DR3 Polyclonal Antibody for WB, ELISA

Cat No. 55397-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

APO 3, APO3, DDR3, Death receptor 3, DR3, LARD, Protein WSL, Protein WSL 1, TNFRSF12, TNFRSF25, TR3, TRAMP, WSL, WSL 1, WSL LR, WSL1

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse spleen tissue, mouse thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

55397-1-AP targets DR3 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor necrosis factor receptor superfamily, member 25
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 52 kDa, 17-20 kDa
• GenBank Accession Number: NM_003790
• Gene Symbol: DR3
• Gene ID (NCBI): 8718
• RRID: AB_11183036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q93038
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFRSF25, also named as APO3, DDR3, DR3, TNFRSF12, WSL, WSL1, LARD, TRAMP and AIR, is a receptor for TNFSF12/APO3L/TWEAK. TNFRSF25 interacts directly with the adapter TRADD. It mediates activation of NF-kappa-B and induces apoptosis. TNFRSF25 may play a role in regulating lymphocyte homeostasis. It is constitutively and highly expressed by CD4(+)FoxP3(+) Tregs. This antibody can recognize all the isoform of TNFRSF25.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Life Sci; TL1A primed dendritic cells activation exacerbated chronic murine colitis.; Authors - Fei Han
human,mouse	WB	Exp Biol Med (Maywood); Silencing of FAM111B inhibits tumor growth and promotes apoptosis by decreasing AKT activity in ovarian cancer; Authors - Wei Wang
mouse	WB	J Ethnopharmacol; Transcriptomic analysis reveals the critical role of Chemokine signaling in the anti-atherosclerosis effect of Xuefu Zhuyu Decoction; Authors - Dongdong Jia