DR3 Polyclonal antibody

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• KD/KO Validated

DR3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55397-1-AP

Synonyms

APO 3, APO3, DDR3, Death receptor 3, DR3, LARD, Protein WSL, Protein WSL 1, TNFRSF12, TNFRSF25, TR3, TRAMP, WSL, WSL 1, WSL LR, WSL1

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Validation Data Gallery

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  WB analysis of PC-3 using 55397-1-AP

  WB result of DR3 antibody (55397-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DR3 transfected PC-3 cells.

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  WB analysis using 55397-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55397-1-AP (DR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 55397-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55397-1-AP (DR3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 55397-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55397-1-AP (DR3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse spleen tissue, mouse thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

55397-1-AP targets DR3 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor necrosis factor receptor superfamily, member 25
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 52 kDa, 17-20 kDa
• GenBank Accession Number: NM_003790
• Gene Symbol: TNFRSF25
• Gene ID (NCBI): 8718
• RRID: AB_11183036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFRSF25, also named as APO3, DDR3, DR3, TNFRSF12, WSL, WSL1, LARD, TRAMP and AIR, is a receptor for TNFSF12/APO3L/TWEAK. TNFRSF25 interacts directly with the adapter TRADD. It mediates activation of NF-kappa-B and induces apoptosis. TNFRSF25 may play a role in regulating lymphocyte homeostasis. It is constitutively and highly expressed by CD4(+)FoxP3(+) Tregs. This antibody can recognize all the isoform of TNFRSF25.

Publications

Species	Application	Title
mouse	WB	Life Sci; TL1A primed dendritic cells activation exacerbated chronic murine colitis.; Authors - Fei Han
human,mouse	WB	Exp Biol Med (Maywood); Silencing of FAM111B inhibits tumor growth and promotes apoptosis by decreasing AKT activity in ovarian cancer; Authors - Wei Wang

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