TNFRSF9/CD137 Polyclonal antibody

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• TNFRSF9/CD137 Polyclonal antibody (32133-1-AP)

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  WB analysis using 32133-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32133-1-AP (4-1BB/TNFRSF9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

TNFRSF9/CD137 Polyclonal Antibody for WB, ELISA

Cat No. 32133-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

T-cell antigen 4-1BB, Ly63, Ila, CD137, 4-1BB ligand receptor

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Tested Applications

• Positive WB detected in: CTLL-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32133-1-AP targets TNFRSF9/CD137 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: tumor necrosis factor receptor superfamily, member 9
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa, 40-45 kDa
• GenBank Accession Number: NM_001077509.1
• Gene Symbol: Tnfrsf9
• Gene ID (NCBI): 21942
• RRID: AB_3670203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P20334
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)