TNFSF13B Polyclonal antibody

TNFSF13B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11862-1-AP

Synonyms

B cell activating factor, B lymphocyte stimulator, BAFF, BLYS, CD257, DTL, TALL 1, TALL1, THANK, TNFSF13B, TNFSF20, ZTNF4

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Validation Data Gallery

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  WB analysis using 11862-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11862-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11862-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11862-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11862-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11862-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U-937 cells, mouse testis tissue, mouse liver tissue, human liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11862-1-AP targets TNFSF13B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFSF13B fusion protein Ag2440
• Full Name: tumor necrosis factor (ligand) superfamily, member 13b
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa, 45-52 kDa
• GenBank Accession Number: BC020674
• Gene Symbol: TNFSF13B
• Gene ID (NCBI): 10673
• RRID: AB_2303658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFSF13B, also known as BAFF, BLYS, TALL1, TNFSF20, ZTNF4 and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. This cytokine is expressed in B lineage cells, and acts as a potent B cell activator. The BAFF system promotes B cell survival and differentiation and plays a prominent role in the pathogenesis of autoimmune diseases. It has been shown that BAFF inhibitors are in clinical trials for systemic lupus erythematosus with significant efficacy. This antibody recognizes all the forms of TNFSF13B such as soluble form (17 kDa), membrane form (32-35 kDa) and glycosylation form (45-52 kDa).