Tested Applications
| Positive WB detected in | COLO 320 cells, SGC-7901 cells, COLO 320, HEK-293 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23899-1-AP targets TNFSF18 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20979 Product name: Recombinant human TNFSF18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-177 aa of BC069319 Sequence: MCLSHLENMPLSHSRTQGAQRSSWKLWLFCSIVMLLFLCSFSWLIFIFLQLETAKEPCMAKFGPLPSKWQMASSEPPCVNKVSDWKLEILQNGLYLIYGQVAPNANYNDVAPFEVRLYKNKDMIQTLTNKSKIQNVGGTYELHVGDTIDLIFNSEHQVLKNNTYWGIILLANPQFIS Predict reactive species |
| Full Name | tumor necrosis factor (ligand) superfamily, member 18 |
| Calculated Molecular Weight | 177 aa, 20 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC069319 |
| Gene Symbol | TNFSF18 |
| Gene ID (NCBI) | 8995 |
| RRID | AB_2879355 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNG2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TNFSF18 (Tumor Necrosis Factor Superfamily Member 18), also known as GITRL (Glucocorticoid-Induced TNF Receptor Ligand) or AITRL (Activation-Induced T Cell Receptor Ligand), is a cytokine belonging to the tumor necrosis factor (TNF) ligand family. TNFSF18 is broadly expressed in various tissues, including the gallbladder, brain, and other tissues. TNFSF18 is expressed on the surface of antigen-presenting cells (APCs) such as dendritic cells, macrophages, and B cells. It is also found in endothelial cells, which play a role in the interaction between T lymphocytes and endothelial cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNFSF18 antibody 23899-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-GITRL antibody (Cat# 23899-1-AP) has 5 total citations published in Cell Death & Disease (2), Cell Death Discovery, Cell Reports, and American Journal of Surgical Pathology. Research fields span hepatology (liver progenitor cell signaling), oncology (colorectal cancer microenvironment, follicular lymphoma), immunology (T-cell regulation), and neuroscience (microglia-mediated astrocyte morphogenesis). Applications include IF, IHC, and WB across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cell Death Dis GITRL impairs hepatocyte repopulation by liver progenitor cells to aggravate inflammation and fibrosis by GITR+CD8+ T lymphocytes in CDE Mice | ||
Cell Death Dis GITR/GITRL reverse signalling modulates the proliferation of hepatic progenitor cells by recruiting ANXA2 to phosphorylate ERK1/2 and Akt. | ||
Cell Death Discov RUNX1 restrains STAT1-GITRL signaling to shape an immunosuppressive CRC microenvironment.
| ||
Cell Rep Microglia mediated by LAG3 regulate astrocyte morphogenesis in the brain white matter.
| ||
Am J Surg Pathol High FCGR2B Expression Can Identify Low-tumor-burden Follicular Lymphoma Patients Who Do Not Require Any Antilymphoma Therapy for a Long Term |
