TNIP2 Polyclonal antibody

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• TNIP2 Polyclonal antibody (15459-1-AP)

TNIP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15459-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

A20-binding inhibitor of NF-kappa-B activation 2, ABIN 2, ABIN2, ABIN-2, Fetal liver LKB1-interacting protein

• 

  WB analysis using 15459-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15459-1-AP (TNIP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 15459-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15459-1-AP (TNIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 15459-1-AP

  IP result of anti-TNIP2 (IP:15459-1-AP, 4ug; Detection:15459-1-AP 1:500) with HepG2 cells lysate 3000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15459-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15459-1-AP (TNIP2 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15459-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15459-1-AP (TNIP2 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 15459-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TNIP2 antibody (15459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, HuH-7 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15459-1-AP targets TNIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7731

  Product name: Recombinant human TNIP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 76-429 aa of BC002740

  Sequence: EQLRRQEGGAAEAQMRQEIERLTERLEEKEREMQQLLSQPQHEREKEVVLLRRSMAEGERARAASDVLCRSLANETHQLRRTLTATAHMCQHLAKCLDERQHAQRNVGERSPDQSEHTDGHTSVQSVIEKLQEENRLLKQKVTHVEDLNAKWQRYNASRDEYVRGLHAQLRGLQIPHEPELMRKEISRLNRQLEEKINDCAEVKQELAASRTARDAALERVQMLEQQILAYKDDFMSERADRERAQSRIQELEEKVASLLHQVSWRQDSREPDAGRIHAGSKTAKYLAADALELMVPGGWRPGTGSQQPEPPAEGGHPGAVQRGQGDLQCPHCLQCFSDEQGEELLRHVAECCQ

• Full Name: TNFAIP3 interacting protein 2
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002740
• Gene Symbol: TNIP2
• Gene ID (NCBI): 79155
• RRID: AB_10859096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NFZ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNIP2, also named as ABIN2 and FLIP1, inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector NEMO/IKBKG. It acts as transcriptional coactivator when translocated to the nucleus. TNIP2 has some isoforms with MW 49 kDa and 37 kDa.

Publications

What published studies show

The TNIP2 Polyclonal antibody (Cat# 15459-1-AP) has 8 citations spanning journals including Nature Communications, Science Signaling, Journal of Virology, Immunology and Inflammation Disease, Neuropeptides, Neuroscience Letters, Journal of Cardiothoracic Surgery, and Molecular Cell. Research fields covered include stem cell biology, inflammation (neutrophilic, endometritis, acute lung injury), virology, neurology (spinal cord injury, amyloidogenesis), and immunology (TNF-driven immunopathology).

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Cell-type-specific functionality encoded within the intrinsically disordered regions of OCT4.; Authors - Burak Ozkan
mouse	WB	Sci Signal; The kinase TPL2 activates ERK and p38 signaling to promote neutrophilic inflammation.; Authors - Kate Senger
pig,mouse	WB	J Virol; Foot-and-Mouth Disease Virus Structural Protein VP1 Destroys the Stability of TPL2 Trimer by Degradation TPL2 to Evade Host Antiviral Immunity.; Authors - Keshan Zhang
human	WB	Immun Inflamm Dis; TNFAIP3 interacting protein 2 relieves lipopolysaccharide (LPS)-induced inflammatory injury in endometritis by inhibiting NF-kappaB activation; Authors - Xinxin Qian
rat	IF	Neuropeptides; Protective effect of TNIP2 on the inflammatory response of microglia after spinal cord injury in rats; Authors - Jiawei Fu
mouse,human	WB,IF	Neurosci Lett; TNIP2 inhibits amyloidogenesis by regulating the 3'UTR of BACE1: an in vitro study; Authors - Long Chen