TNNI1 Polyclonal antibody

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  WB analysis of human skeletal muscle using 16102-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16102-1-AP (TNNI1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 16102-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16102-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 16102-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16102-1-AP (TNNI1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 16102-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16102-1-AP (TNNI1 Antibody) at dilution of 1:50 (under 40x lens).

TNNI1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16102-1-AP

Publications(7)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

SSTNI, TNN1, TNNI1

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissues
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 2 publications below

Product Information

16102-1-AP targets TNNI1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9001

  Product name: Recombinant human TNNI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-187 aa of BC012600

  Sequence: MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVMDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

• Full Name: troponin I type 1 (skeletal, slow)
• Calculated Molecular Weight: 187 aa, 22 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC012600
• Gene Symbol: TNNI1
• Gene ID (NCBI): 7135
• RRID: AB_2206103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19237
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB,IF	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
human	WB	iScience; Engineering of thick human functional myocardium via static stretching and electrical stimulation.; Authors - Danielle Pretorius
mouse	WB	Sci Rep; A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice.; Authors - Kensuke Ihara
mouse	WB	Front Physiol; Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle.; Authors - William J Valentine
human	WB	Cell Death Discov; Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.; Authors - Bin Zhou
mouse	IF	Front Med; Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart; Authors - Miaomiao Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xuebin (Verified Customer) (11-12-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: heart