TNNI1 Polyclonal antibody

TNNI1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16102-1-AP

Synonyms

SSTNI, TNN1, TNNI1



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissues
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16102-1-AP targets TNNI1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNNI1 fusion protein Ag9001
Full Name troponin I type 1 (skeletal, slow)
Calculated molecular weight 187 aa, 22 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC012600
Gene symbol TNNI1
Gene ID (NCBI) 7135
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TNNI1 antibody 16102-1-APDownload protocol
IHC protocol for TNNI1 antibody 16102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Basic Res Cardiol

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Authors - Alzbeta Hulikova
humanWB

iScience

Engineering of thick human functional myocardium via static stretching and electrical stimulation.

Authors - Danielle Pretorius
mouseWB

Sci Rep

A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice.

Authors - Kensuke Ihara
mouseWB

Front Physiol

Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle.

Authors - William J Valentine
humanWB

Cell Death Discov

Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.

Authors - Bin Zhou
mouseIF

Front Med

Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart

Authors - Miaomiao Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xuebin (Verified Customer) (11-12-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: heart