TNNI1 Polyclonal antibody

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• TNNI1 Polyclonal antibody (16102-1-AP)

TNNI1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 16102-1-AP Copied!

Publications (8)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

SSTNI, TNN1, Troponin I, slow-twitch isoform

• 

  WB analysis of human skeletal muscle using 16102-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16102-1-AP (TNNI1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 16102-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16102-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 16102-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16102-1-AP (TNNI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 16102-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16102-1-AP (TNNI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse skeletal muscle using 16102-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TNNI1 antibody (16102-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissues
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16102-1-AP targets TNNI1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9001

  Product name: Recombinant human TNNI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-187 aa of BC012600

  Sequence: MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVMDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

• Full Name: troponin I type 1 (skeletal, slow)
• Calculated Molecular Weight: 187 aa, 22 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC012600
• Gene Symbol: TNNI1
• Gene ID (NCBI): 7135
• RRID: AB_2206103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19237
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TNNI1 Polyclonal antibody (catalog number 16102-1-AP) has 8 total citations. These appear in journals including Signal Transduction and Targeted Therapy, Cell Stem Cell, Cell Death Discovery, Basic Research in Cardiology, Frontiers in Medicine, Scientific Reports, iScience, and Frontiers in Physiology. The associated research fields span cardiac biology and cardiomyocyte development, stem cell-derived cardiomyocytes, thyroid cancer, dilated cardiomyopathy, atrial fibrillation, and muscle lipidomics, with applications primarily in Western blot and immunofluorescence.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; Thiamine-modified metabolic reprogramming of human pluripotent stem cell-derived cardiomyocyte under space microgravity; Authors - Xinglong Han
		Cell Stem Cell; Human PSC-derived sinoatrial node-cardiac plexus assembloids model innervation-associated maturation of pacemaker systems.; Authors - Tongdong Zhang
human	WB	Cell Death Discov; Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.; Authors - Bin Zhou
rat	WB,IF	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
mouse	IF	Front Med; Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart; Authors - Miaomiao Zhu
mouse	WB	Sci Rep; A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice.; Authors - Kensuke Ihara

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

The single available review for this product rates it 4 out of 5 stars. The reviewer used it at a 1:50 dilution for immunofluorescence on heart tissue. No written comments were provided.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Xuebin (Verified Customer) (11-12-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: heart