TNNI1 Polyclonal antibody

TNNI1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 22253-1-AP

Synonyms

SSTNI, TNN1, TNNI1



Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22253-1-AP is TNNI1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNNI1 fusion protein Ag17749
Full Name troponin I type 1 (skeletal, slow)
Calculated molecular weight 187 aa, 22 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC012600
Gene symbol TNNI1
Gene ID (NCBI) 7135
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TNNI1 antibody 22253-1-APDownload protocol
IHC protocol for TNNI1 antibody 22253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols