TNNI1 Polyclonal antibody

• 

  WB analysis using 22253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22253-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 22253-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22253-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 22253-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22253-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens).

TNNI1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22253-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SSTNI, TNN1, TNNI1

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22253-1-AP targets TNNI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNNI1 fusion protein Ag17749
• Full Name: troponin I type 1 (skeletal, slow)
• Calculated Molecular Weight: 187 aa, 22 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC012600
• Gene Symbol: TNNI1
• Gene ID (NCBI): 7135
• RRID: AB_11182276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19237
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.