TNNI1 Polyclonal antibody

TNNI1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 22253-1-AP

Synonyms

SSTNI, TNN1, TNNI1

---

Validation Data Gallery

• 

  WB analysis using 22253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22253-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 22253-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22253-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 22253-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22253-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22253-1-AP is TNNI1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNNI1 fusion protein Ag17749
• Full Name: troponin I type 1 (skeletal, slow)
• Calculated molecular weight: 187 aa, 22 kDa
• Observed molecular weight: 25 kDa
• GenBank accession number: BC012600
• Gene symbol: TNNI1
• Gene ID (NCBI): 7135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.