TNNI2 Polyclonal antibody

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• TNNI2 Polyclonal antibody (11634-1-AP)

TNNI2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11634-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AMCD2B, DA2B, FSSV, fsTnI, TNNI2, Troponin I fast skeletal muscle

• 

  WB analysis using 11634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 11634-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat skeletal muscle using 11634-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human kidney using 11634-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human spleen using 11634-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human skeletal muscle using 11634-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11634-1-AP (TNNI2 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11634-1-AP (TNNI2 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human kidney tissue, human spleen tissue, human skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11634-1-AP targets TNNI2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2208

  Product name: Recombinant human TNNI2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-182 aa of BC032148

  Sequence: MGDEEKRNRAITARRQHLKSVMLQIAATELEKEESRREAEKQNYLAEHCPPLHIPGSMSEVQELCKQLHAKIDAAEEEKYDMEVRVQKTSKELEDMNQKLFDLRGKFKRPPLRRVRMSADAMLKALLGSKHKVCMDLRANLKQVKKEDTEKERDLRDVGDWRKNIEEKSGMEGRKKMFESES

• Full Name: troponin I type 2 (skeletal, fast)
• Calculated Molecular Weight: 182 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC032148
• Gene Symbol: TNNI2
• Gene ID (NCBI): 7136
• RRID: AB_2206109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48788
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TNNI2 Polyclonal antibody (Cat# 11634-1-AP) has 1 total citation. It appears in the journal Heliyon (Impact Factor: 4), in a 2024 study by Fangping Lu et al. The research investigates how exogenous hydrogen sulfide enhances myogenic differentiation of C2C12 myoblasts under high palmitate stress, placing it in the fields of muscle biology and metabolic stress responses. The antibody was used for Western blot (WB) analysis in mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Heliyon; Exogenous hydrogen sulfide enhances myogenic differentiation of C2C12 myoblasts under high palmitate stress; Authors - Fangping Lu