TNNT3 Polyclonal antibody

TNNT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19729-1-AP

Synonyms

Troponin T-FS, Troponin T3 skeletal fast, Fast skeletal muscle troponin T, Beta-TnTF

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Validation Data Gallery

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  WB analysis of rat skeletal muscle using 19729-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19729-1-AP (TNNT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 19729-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19729-1-AP (TNNT3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 19729-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 19729-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 19729-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19729-1-AP (TNNT3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 19729-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19729-1-AP (TNNT3 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: rat skeletal muscle tissue, mouse skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19729-1-AP targets TNNT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: troponin T type 3 (skeletal, fast)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: NM_001042780
• Gene Symbol: TNNT3
• Gene ID (NCBI): 7140
• RRID: AB_2878601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.