TNPO2 Polyclonal antibody

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• KD/KO Validated

TNPO2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17831-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, CoIP, ELISA

Synonyms

TNPO 2, KPNB2B, Karyopherin beta-2b, IPO3, Importin 3

• 

  WB analysis of fetal human brain using 17831-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 17831-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 17831-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 17831-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 17831-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 17831-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TNPO2 antibody (17831-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: fetal human brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17831-1-AP targets TNPO2 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12229

  Product name: Recombinant human TNPO2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC072420

  Sequence: MDWQPDEQGLQQVLQLLKDSQSPNTATQRIVQDKLKQLNQFPDFNNYLIFVLTRLKSEDEPTRSLSGLILKNNVKAHYQSFPPPVADFIKQECLNNIGDASSLIRATIGILITTIASKGELQMWPELLPQLCNLLNSEDYNTCEGAFGALQKICEDSSELLDSDALNRPLNIMIPKFLQFFKHCSPKIRSHAIACVNQFIMDRAQALMDNIDTFIEHLFALAVDDDPEVRKNVCRALVMLLEVRIDRLIPHMHSIIQYMLQRTQDHDENVALEACEFWLTLAEQPICKEVLASHLVQLIPILVNGMKYSEIDIILLKGDVEEDEAVPDSEQDIKPRFHKSRTVTLPHEAE

• Full Name: transportin 2
• Calculated Molecular Weight: 887 aa, 100 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC072420
• Gene Symbol: TNPO2
• Gene ID (NCBI): 30000
• RRID: AB_10598481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14787
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TNPO2 Polyclonal antibody (Cat# 17831-1-AP) has 5 citations published in Int J Biol Macromol, Cell Reports, Scientific Reports, J Biol Chem, and eLife. The associated research fields include glioblastoma cell motility, neuronal FUS cytoplasmic translocation under hypertonic stress, oestrogen receptor-α nucleocytoplasmic shuttling, circadian rhythm regulation via CHRONO nuclear transport, and β-catenin nuclear transport in Wnt signaling.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CoIP	Int J Biol Macromol; Membrane-bound transcription factor LRRC4 inhibits glioblastoma cell motility; Authors - Yang Liu
human	IF	Cell Rep; Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.; Authors - Eva-Maria Hock
human	WB,IF	Sci Rep; Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-α.; Authors - Tetsuji Moriyama ; KD Validated
human	WB	J Biol Chem; The nuclear transportation of CHRONO regulates the circadian rhythm; Authors - Qin Zhou
mouse	WB	eLife; Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling; Authors - Woong Y Hwang