TNS4 Polyclonal antibody

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  WB analysis using 11580-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11580-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 11580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 11580-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11580-1-AP (TNS4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

TNS4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11580-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Tensin-4, tensin 4, PP14434, C-terminal tensin-like protein, CTEN

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Tested Applications

• Positive WB detected in: HepG2 cells, human placenta tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11580-1-AP targets TNS4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2169

  Product name: Recombinant human TNS4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 130-480 aa of BC018706

  Sequence: ITNSMVDIPIVLINGCPEPGSSPPQRTPGHQNSVQPGAASPSNPCPATRSNSQTLSDAPFTTCPEGPARDMQPTMKFVMDTSKYWFKPNITREQAIELLRKEEPGAFVIRDSSSYRGSFGLALKVQEVPASAQSRPGEDSNDLIRHFLIESSAKGVHLKGADEEPYFGSLSAFVCQHSIMALALPCKLTIPQRELGGADGASDSTDSPASCQKKSAGCHTLYLSSVSVETLTGALAVQKAISTTFERDILPTPTVVHFKVTEQGITLTDVQRKVFFRRHYPLTTLRFCGMDPEQRKWQKYCKPSWIFGFVAKSQTEPQENVCHLFAEYDMVQPASQVIGLVTALLQDAERM

• Full Name: tensin 4
• Calculated Molecular Weight: 715 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC018706
• Gene Symbol: TNS4
• Gene ID (NCBI): 84951
• RRID: AB_10640582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZW8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNS4 (Tensin-4), also known as COOH-terminus tensin-like protein, is a distinctive member with the smallest molecular mass and lacks the N-terminal common region found in other family members. TNS4 plays a crucial role in CRC tumorigenesis, and that suppression of TNS4 would be an effective therapeutic strategy in treating a subset of cetuximab-refractory CRC patients including KRAS activating mutations (PMID: 31409052). TNS4 has been implicated in numerous vital biological processes, including proliferation, migration, cell adhesion, and apoptosis (PMID: 38164166).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Front Bioeng Biotechnol; Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness.; Authors - Elizabeth M Boazak
human	WB,IHC	Oncol Lett; Tensin 4 facilitates aerobic glycolysis, migration and invasion of colorectal cancer cells through the β‑catenin/c‑Myc signaling pathway; Authors - Yan Wang ; KD Validated
human	WB	Pathol Res Pract; Tensin4 promotes invasion and migration of gastric cancer cells via regulating AKT/GSK-3β/snail signaling pathway.; Authors - Xiumin Qi ; KD Validated
human	IHC	Front Immunol; Development and functional validation of a disulfidoptosis-related gene prognostic model for lung adenocarcinoma based on bioinformatics and experimental validation; Authors - Tao Shen
human,mouse	WB,IP,IHC	Cancer Cell Int; TNS4 promotes lymph node metastasis of gastric cancer by interacting with integrin Β1 and inducing the activation of fibroblastic reticular cell; Authors - Xiang Zhang ; KD Validated
human	WB	Antioxid Redox Signal; A novel lncRNA FUAT1/TNS4 axis confers chemoresistance by suppressing ROS-mediated apoptosis in gastric cancer; Authors - Mingliang Liu