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- KD/KO Validated
TNS4 Polyclonal antibody
TNS4 Polyclonal Antibody for WB, IF, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, FC, IHC, CoIP, ELISA, Cell treatment
Conjugate
Unconjugated
Cat no : 11580-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, human placenta tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A549 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
CoIP | See 1 publications below |
Product Information
11580-1-AP targets TNS4 in WB, IF, FC, IHC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TNS4 fusion protein Ag2169 |
Full Name | tensin 4 |
Calculated Molecular Weight | 715 aa, 77 kDa |
Observed Molecular Weight | 77 kDa |
GenBank Accession Number | BC018706 |
Gene Symbol | TNS4 |
Gene ID (NCBI) | 84951 |
RRID | AB_10640582 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for TNS4 antibody 11580-1-AP | Download protocol |
IHC protocol for TNS4 antibody 11580-1-AP | Download protocol |
IF protocol for TNS4 antibody 11580-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Bioeng Biotechnol Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness. | ||
Pathol Res Pract Tensin4 promotes invasion and migration of gastric cancer cells via regulating AKT/GSK-3β/snail signaling pathway.
| ||
Antioxid Redox Signal A novel lncRNA FUAT1/TNS4 axis confers chemoresistance by suppressing ROS-mediated apoptosis in gastric cancer | ||
Int J Biol Sci Hypoxia-driven TNS4 fosters HNSCC tumorigenesis by stabilizing integrin α5β1 complex and triggering FAK-mediated Akt and TGFβ signaling pathways |