TOAG1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TOAG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21564-1-AP

Synonyms

C3orf23, TCAIM, T-cell activation inhibitor, TOAG1

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Validation Data Gallery

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  WB analysis of HEK-293 using 21564-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21564-1-AP (TOAG1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21564-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21564-1-AP (TOAG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 21564-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21564-1-AP (TOAG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Product Information

21564-1-AP targets TOAG1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOAG1 fusion protein Ag16067
• Full Name: chromosome 3 open reading frame 23
• Calculated Molecular Weight: 496 aa, 58 kDa
• Observed Molecular Weight: 35-41 kDa
• GenBank Accession Number: BC101060
• Gene Symbol: TOAG1
• Gene ID (NCBI): 285343
• RRID: AB_2878885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOAG1, also named as TCAIM and C3orf23, is a mitochondrial protein which regulates T-cell apoptosis. It is a specific and reproducible marker for quiescence. TOAG1 has two isoforms with MW 41 kDa and 58 kDa.

Publications

Species	Application	Title
human	IHC	FEBS J; A novel role of TCAIM: suppressing renal carcinoma growth and enhancing its sensitivity to sunitinib; Authors - Zhuo Ye ; KD Validated