Tom22 Monoclonal antibody

Tom22 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human and More (2)







Cat no : 66562-1-Ig


1C9 2, hTom22, MST065, MSTP065, TOM22, TOMM22

Tested Applications

Positive WB detected inA431 cells, T-47D cells, L02 cells, Raji cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66562-1-Ig targets Tom22 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Tom22 fusion protein Ag1797
Full Name translocase of outer mitochondrial membrane 22 homolog (yeast)
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009363
Gene Symbol TOMM22
Gene ID (NCBI) 56993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).


Product Specific Protocols
WB protocol for Tom22 antibody 66562-1-IgDownload protocol
IHC protocol for Tom22 antibody 66562-1-IgDownload protocol
IF protocol for Tom22 antibody 66562-1-IgDownload protocol
FC protocol for Tom22 antibody 66562-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang

Nat Commun

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Authors - Wenjie Bao