CoraLite®594-conjugated Tom22 Monoclonal antibody

Tom22 Monoclonal Antibody for FC (Intra), WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C2B11

Cat no : CL594-66562

Synonyms

1C9 2, hTom22, MST065, MSTP065, TOM22, TOMM22

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Validation Data Gallery

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  WB analysis using CL594-66562

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66562 (TOMM22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using CL594-66562

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Tom22 (CL594-66562, Clone:1C2B11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66562 targets Tom22 in WB, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Tom22 fusion protein Ag1797
• Full Name: translocase of outer mitochondrial membrane 22 homolog (yeast)
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC009363
• Gene Symbol: TOMM22
• Gene ID (NCBI): 56993
• RRID: AB_2920005
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOMM22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C