CoraLite®594-conjugated TOMM22 Monoclonal antibody

TOMM22 Monoclonal Antibody for FC (Intra), WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C2B11

Cat no : CL594-66562

Synonyms

1C9 2, hTom22, MST065, MSTP065, TOM22, TOMM22



Tested Applications

Positive WB detected inHeLa cells
Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66562-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

CL594-66562 targets TOMM22 in WB, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TOMM22 fusion protein Ag1797
Full Name translocase of outer mitochondrial membrane 22 homolog (yeast)
Calculated molecular weight 15 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009363
Gene symbol TOMM22
Gene ID (NCBI) 56993
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOMM22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

Protocols

Product Specific Protocols
WB protocol for CL594 TOMM22 antibody CL594-66562Download protocol
FC protocol for CL594 TOMM22 antibody CL594-66562Download protocol
Standard Protocols
Click here to view our Standard Protocols